Cloning vectors for homologous recombination and method...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/79 (2006.01) C12N 15/70 (2006.01)

Patent

CA 2448983

The invention concerns novel vectors for use both in creating genomic DNA banks and as vectors for carrying out homologous recombination reactions in host cells, in particular for improving selection of said homologous recombination events, and decreasing the time for obtaining a final vector for performing the homologous recombination reaction. The invention also concerns a method using said vectors.

La présente invention se rapporte à des nouveaux vecteurs utilisables à la fois pour créer des banques d'ADN génomiques et en tant que vecteurs pour la réalisation de réactions de recombinaison homologue dans des cellules hôtes, permettant notamment d'améliorer la sélection desdits événements de recombinaison homologue, et de diminuer le temps d'obtention du vecteur final pour effectuer la réaction de recombinaison homologue. L'invention a également pour objet un procédé mettant en oeuvre ces vecteurs.

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