C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) C12N 9/02 (2006.01) C12N 15/52 (2006.01)
Patent
CA 2332615
This invention provides improved monoxygenases, dehydrogenases, and transferases that are useful for the biocatalytic synthesis of compounds such as .alpha.-hydroxycarboxylic acids, and aryl- and alkyl-, hydroxy compounds. The polypeptides provided herein are improved in properties such as regioselectivity, enzymatic activity, stereospecificity, and the like. Methods for obtaining recombinant polynucleotides that encode these improved polypeptides are also provided, as are organisms that express the polypeptides and are thus useful for carrying out said biocatalytic syntheses. Also provided by the invention are methods for increasing said solvent resistance of organisms that are used in the synthetic methods.
L'invention concerne des monoxygénases, des déshydrogénases et des transférases améliorées, qui sont utiles pour la synthèse biocatalytique de composés tels que des acides hydroxycarboxyliques .alpha. et des composés aryle, alkyle et hydroxy. Les polypeptides en question sont améliorés en ce qui concerne des propriétés telles que régiosélectivité, activité enzymatique, stéréospécificité et analogue. L'invention concerne également des procédés permettant d'obtenir des polynucléotides recombinés qui codent pour ces polypeptides améliorés, ainsi que des organismes qui expriment les polypeptides et sont de ce fait utiles pour mettre en oeuvre lesdites synthèses biocatalytiques. L'invention concerne également des procédés permettant d'accroître la résistance aux solvants desdits organismes qui sont utilisés dans les procédés de synthèse.
Affholter Joseph A.
Davis S. Christopher
Selifonov Sergey A.
Fetherstonhaugh & Co.
Maxygen Inc.
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