Improved nucleic acid amplification

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) C07H 21/04 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01) C12P 21/06 (2006.01)

Patent

CA 2477670

The present invention provides improved methods for the amplification of nucleic acid molecules. Methods for amplifying target polynucleotides, including mRNA, using oligonucleotides, DNA and RNA polymerases are provided. The invention further provides compositions and kits for practicing the methods, as well as methods which use the amplification products.

L'invention concerne des procédés améliorés d'amplification de molécules d'acides nucléiques. Elle concerne aussi des procédés d'amplification de polynucléotides cibles, y compris d'ARNm, au moyen d'oligonucléotides et de polymérases d'ARN et d'ADN. Elle concerne enfin des compositions et des kits permettant la mise en oeuvre des procédés, ainsi que des procédés utilisant les produits d'amplification.

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