Oxidized styrenic polymers for dna binding

Details Oxidized styrenic polymers for dna binding Oxidized styrenic polymers for dna binding

C - Chemistry, Metallurgy

07

H

C07H 21/00 (2006.01) C08G 63/91 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2297745

A method of immobilizing aminated DNA to a substrate surface is provided for use in DNA hybridization assays. The surface can be widely employed in a variety of molded laboratory products including multiwell plates, cell culture dishes, and biological containers. The method comprises the steps of providing a polystyrene substrate having a surface content of between 0.3 and 4.0 % atomic percent oxygen, and attaching amine modified oligonucleotide to the surface of the substrate. A resultant product comprising a polystrene substrate having a surface oxygen content of between 0.3 and 4.0 % atomic percent, and a plurality of amine modified oligonucleotides directly attached to the surface of the substrate, is also disclosed.

L'invention concerne un procédé d'immobilisation d'ADN aminé sur une surface de substrat en vue de l'utilisation dans des dosages d'hybridation d'ADN. La surface peut être largement employée dans divers produits de laboratoire moulés, notamment dans des plaques à alvéoles multiples, des récipients plats de culture cellulaire et des récipients biologiques. Le procédé consiste à prendre un substrat en polystyrène dont la teneur en surface en oxygène est comprise entre 0,3 et 4,0 % en pourcentage atomique, et à fixer des oligonucléotides modifiés par amine à la surface du substrat. L'invention concerne également un produit ainsi obtenu qui comprend un substrat en polystyrène dont la teneur en surface en oxygène est comprise entre 0,3 et 4,0 % en pourcentage atomique, ainsi que plusieurs oligonucléotides modifiés par amine et directement fixés à la surface du substrat.

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