Process for the specific detection of glycosylated proteins

Details Process for the specific detection of glycosylated proteins Process for the specific detection of glycosylated proteins

G - Physics

01

N

G01N 33/566 (2006.01) G01N 33/53 (2006.01) G01N 33/577 (2006.01) G01N 33/58 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2291458

A process for the specific detection of glycosylated proteins and an assay for carrying out this detection process is described, in which a) incubating the sample to be investigated with a solid phase which is coated with a substrate which immobilizes the glycosidic groups bonded to the protein, b) removing the remains of the sample not bound to the solid phase by washing, c) allowing a labeled F(ab) fragment or F(ab')2 fragment of a monoclonal antibody directed against the specific protein to act on the immobilized, glycosylated protein, and d) after washing out the unbound F(ab) fragment or F(ab')2 fragment, detecting the bound fraction.

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