Methods and compositions for expressing negative-sense viral...

Details Methods and compositions for expressing negative-sense viral... Methods and compositions for expressing negative-sense viral...

C - Chemistry, Metallurgy

12

N

C12N 15/85 (2006.01) C12N 5/10 (2006.01) C12N 7/00 (2006.01) C12N 7/01 (2006.01) C12N 15/11 (2006.01) C12N 15/86 (2006.01) C12N 15/44 (2006.01)

Patent

CA 2646526

The present invention provides novel canine pol I regulatory nucleic acid sequences useful for the expression of nucleic acid sequences in canine cells such as MDCK cells. The invention further provides expression vectors and cells comprising such nucleic acids as well as methods of using such nucleic acids to make influenza viruses, including infectious influenza viruses.

L'invention porte sur une nouvelle séquence d'acides nucléiques régulatrice de la pol I canine provoquant l'expression de séquences d'acides nucléiques dans des cellules canines telles que les cellules MDCK. L'invention porte également sur des vecteurs d'expression et des cellules comprenant lesdits acides nucléiques et sur des méthodes d'utilisation desdits acides nucléiques pour produire des virus de la grippe y compris des virus de la grippe infectieuse.

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