Use and production of citrate-stable neutral metalloproteases

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 9/54 (2006.01)

Patent

CA 2703975

The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved stability in the presence of a metal chelator. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications comprising citrate. In some particularly preferred embodiments, the present invention provides methods and compositions comprising variant neutral metalloprotease(s) engineered to resist citrate-induced autolysis.

La présente invention porte sur des procédés et des compositions comprenant au moins une enzyme métalloprotéase neutre qui présente une stabilité améliorée en présence d'un chélateur de métaux. Dans certains modes de réalisation, la métalloprotéase neutre trouve une utilisation dans des applications de nettoyage et autres applications comprenant du citrate. Dans certains modes de réalisation particulièrement privilégiés, la présente invention porte sur des procédés et des compositions comprenant une ou des métalloprotéases neutres variantes mises au point pour résister à une autolyse induite par un citrate.

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