Diagnostics based on tetrazolium compounds

Details Diagnostics based on tetrazolium compounds Diagnostics based on tetrazolium compounds

C - Chemistry, Metallurgy

12

Q

C12Q 1/54 (2006.01) C12Q 1/26 (2006.01) G01N 33/52 (2006.01) G01N 33/72 (2006.01)

Patent

CA 2337562

A reagent is suitable for measuring the concentration of an analyte in a hemoglobin-containing biological fluid, such as whole blood. The reagent comprises a flavin- dependent enzyme that has specificity for the analyte, a flavin cofactor if, and only if, a flavin is not bound to the enzyme, a tetrazolium dye precursor, an electron transfer agent, and a nitrite salt. The reagent causes dye formation that is a measure of the analyte concentration. The nitrite salt suppresses interfering dye formation caused non-enzymatically by the hemoglobin. Preferably, the reagent is used in a dry strip for measuring glucose in whole blood.

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