In vitro maturation of denuded bovine oocytes

Details In vitro maturation of denuded bovine oocytes In vitro maturation of denuded bovine oocytes

C - Chemistry, Metallurgy

12

N

C12N 5/06 (2006.01) A01K 67/02 (2006.01) C12N 5/02 (2006.01)

Patent

CA 2072475

-23- IN VITRO MATURATION OF DENUDED BOVINE OOCYTES ABSTRACT The present invention is directed to a process for maturing denuded bovine oocytes. The process includes stripping cumulus cells from the oocyte and allowing the denuded oocyte to mature in a conditioned medium including maintenance medium having therein a monolayer of cumulus cells for a period of approximately 10-24 hours, and preferably 16-20 hours. The process can also be adapted to an oocyte screening process for selecting bovine oocytes which make better candidates for fertilization and embryo development. The process includes observing the development and ejection of the polar body. This phenomenon has direct correlation with the developmental potential of the bovine oocyte.

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