Pna-dna-pna chimeric macromolecules

C - Chemistry – Metallurgy – 07 – K

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C07K 14/00 (2006.01) A61K 38/00 (2006.01) A61K 38/46 (2006.01) C07H 19/04 (2006.01) C07H 21/00 (2006.01) C07K 2/00 (2006.01) C07K 9/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2177357

Macromolecules are provided that have increased nuclease resistance, increasing binding affinity to a complementary strand, and that activate RNase H enzyme. The macromolecules have the structure PNA-DNA-PNA where the DNA portion is composed of subunits of 2'-deoxy-erythro-pentofuranosyl nucleotides and the PNA portions are composed of subunits of peptide nucleic acids. Such macromolecules are useful for diagnostics and other research purposes, for modulating protein in organisms, and for the diagnosis, detection and treatment of other conditions susceptible to therapeutics.

L'invention concerne des macromolécules qui présentent une résistance accrue à la nucléase, intensifiant leur affinité de liaison à un brin complémentaire et qui activent l'enzyme RNase H. Ces macromolécules ont la structure PNA-DNA-PNA dans laquelle la partie DNA est constituée de sous-unités de nucléotides 2'-désoxy-<u>érythro</u>-pentofuranosyle et les parties PNA sont constituées de sous-unités d'acides nucléiques peptidiques. Ces macromolécules sont utiles dans des diagnostics et à d'autres fins de recherche, pour moduler des protéines dans des organismes, et dans le diagnostic, la détection et le traitement d'autres états sensibles à une thérapeutique.

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