G - Physics – 01 – N
Patent
G - Physics
01
N
167/44
G01N 33/569 (2006.01) C07K 14/15 (2006.01) G01N 33/543 (2006.01)
Patent
CA 2025019
The present invention relates to a method for the detection HTLV-1 reactive antibodies and diagnosis of ATL (adult T cell leukemia/ lypmphoma) condition by the use of chemically synthesized peptide compositions. The peptide compositions comprise peptides having amino acid sequences corresponding to transmembrane and external segments of the envelope protein of HTLV-1 acid mixtures thereof. The peptide compositions are highly immunoreactive with antibodies in sera of patients with ATL. The present invention further relates to a method for the simultaneous detection and diagnosis of ATL and Acquired Immune Deficiency Syndrome (AIDS) by the use of chemically synthesized HTLV-1 peptide compositions in conjunction with a chemically synthesized HIV (1 and 2) peptide composition. The detection method includes an enzyme-linked immunosorbent assay (ELISA), an immunoradiometric assay (IRMA), and other forms of immunoassay procedures such as enzyme immuno blotting assay on nitrocellulose paper and hemagglutination assay using the peptide composition as the antigen. The preferred detection method is ELISA.
Macrae & Co.
United Biomedical Inc.
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