Frozen tissue microarrays and methods for using the same

G - Physics – 01 – N

Patent

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G01N 1/42 (2006.01) B01J 19/00 (2006.01) C12Q 1/68 (2006.01) G01N 33/574 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2414062

The invention provides microarrays comprising a plurality of frozen tissues and/or cell samples and methods of preparing and using the same. By using frozen samples, the microarrays provide optimal samples from which to detect the expression of both nucleic acids (e.g., mRNAs) and proteins in high throughput parallel analyses. The microarrays enable gene identification, molecular profiling, selection of promising drug targets, sorting and prioritizing of expressed sequence array data, and the identification of abnormal physiological processes associated with disease.

L'invention concerne des jeux ordonnés de microéchantillons comportant plusieurs tissus congelés et/ou des échantillons de cellules et des procédés de préparation et d'utilisation de ceux-ci. L'emploi d'échantillons congelés permet aux jeux ordonnés de microéchantillons de fournir des échantillons optimaux à partir desquels on peut détecter l'expression des acides nucléiques (par exemple, les mRNAs) et des protéines dans des analyses parallèles à haute capacité. Les jeux ordonnés de microéchantillons permettent également 'identifier des gènes, d'établir des profils moléculaires, de sélectionner des cibles de médicaments intéressantes, de trier et d'attribuer une priorité de données de jeu ordonné de séquence exprimée et d'identifier des procédés physiologiques anormaux associés à des affections.

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