Method for producing mature reconstituted mammalian eggs by...

Details Method for producing mature reconstituted mammalian eggs by... Method for producing mature reconstituted mammalian eggs by...

C - Chemistry, Metallurgy

12

N

C12N 5/16 (2006.01) A01K 67/027 (2006.01) A61D 19/04 (2006.01)

Patent

CA 2059199

ABSTRACT OF THE DISCLOSURE A method for producing mature reconstituted unfertilized mammalian eggs by transferring to enucleated ovulated or in vitro matured eggs nuclei from other sources, includes the following steps: 1) production of the enucleated mature egg or -egg fragment. 2) isolation of the cell or cell fragment containing the nucleus to be transferred. 3) fusion of the enucleated egg or -egg fragment with the cell or cell fragment containing the nucleus to be transferred. Nuclei from a variety of sources may be used including immature and mature eggs, and cells from embryos. The reconstituted eggs may be fertilized by exposure to sperm in vitro or by activation and pronuclear transplantation.

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