C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 14/37 (2006.01) A01H 5/00 (2006.01) A01N 65/00 (2009.01) C12N 15/10 (2006.01) C12N 15/31 (2006.01) C12N 15/82 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2440424
It is intended to search for and identify a novel antimicrobial protein whereby the growth of pathogenic bacteria for plants (for example, Pyricularia orizae and Rhizoctonia solani causing two major diseases of rice plant) at a relatively low concentration and to clone the gene of this protein. Thus, a novel antimicrobial protein which can be obtained from a fraction obtained from a water extract of Pleurotus cornucopiae by the ammonium sulfate precipitation method, has an antimicrobial activity at least against P. orizae and R. solani, and shows the presence of an about 15 kDa component in SDS-PAGE is provided.
L'objectif selon l'invention est de rechercher et d'identifier une nouvelle protéine antimicrobienne permettant d'inhiber la croissance de bactéries pathogènes chez les plantes (par exemple, <i>Pyricularia orizae</i> et <i>Rhizoctonia solani</i> qui entraînent deux graves maladies des cultures de riz) à une concentration relativement faible et de cloner cette protéine. Par conséquent, une nouvelle protéine antimicrobienne, qui peut être obtenue à partir d'une fraction elle-même obtenue d'un extrait d'eau de <i>Pleurotus cornucopiae</i> par un procédé de précipitation de sulfate d'ammonium, présente une activité antimicrobienne contre au moins <i>P. orizae</i> et <i>R. solani</i> et révèle la présence d'un composant d'environ 15 kDa dans SDS-PAGE.
Japan Tobacco Inc.
Robic
Syngenta Limited
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