Device and method for conducting immunoassays

Details Device and method for conducting immunoassays Device and method for conducting immunoassays

G - Physics

01

N

G01N 33/558 (2006.01) G01N 37/00 (2006.01)

Patent

CA 2073504

An immunoassay method comprising applying an aqueous solution containing the analyte antigen to one end of a multi- zoned test strip device (10) such that the solution moves along the strip (10) by capillary action. The zones are arranged so that the solution (a) first contacts and reconstitutes dry, diffusible labelled component comprising colloidal gold conjugated to an antib- ody specific for said analyte antigen (20) and then (b) contacts and reconstitutes dry, diffusible biotinylated second antibody spe- cific for said analyte antigen (18) such that a diffusible, dispersed sandwich reaction product forms. The reaction product diffuses along the strip (10) with the solution and into a zone containing capture component consisting of a latex and avidin complex (22) which avidin collects the reaction product by means of reaction with its biotin moiety. Thus, gold particles are collected and con- centrated in the detection zone (22) for visual determination.

Affiliated with

Also associated with

Rating

Device and method for conducting immunoassays does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Device and method for conducting immunoassays, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Device and method for conducting immunoassays will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1463282