A method for producing procollagen

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/12 (2006.01) A61K 48/00 (2006.01) C07K 14/78 (2006.01) C12N 5/10 (2006.01) C12N 15/62 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2282719

A method of producing a desired procolagen or derivative thereof in a system which co-expresses and assembles at least one further procollagen or derivative thereof. The gene(s) for expressing pro-.alpha. chains or derivatives thereof for assembly into the desired procollagen has or have been exogenously selected from natural pro-.alpha. chains or exogenously manipulated such as to express said pro-.alpha. chains or derivatives thereof with domains which have the activity of C-terminal propeptide domains but which will not co-assemble with the C-terminal propeptide of the pro-.alpha. chains or derivatives thereof that assemble to form the said at least one further procollagen or derivative thereof.

L'invention porte sur un procédé de production d'un procollagène ou de son dérivé dans un système qui coexprime et associe au moins un autre procollagène ou son dérivé. Le(s) gène(s), employés pour exprimer des chaînes pro-alpha ou leurs dérivés qu'on associe pour obtenir le collagène désiré, a ou ont fait l'objet d'une sélection exogène à partir de chaînes pro-alpha naturelles, ou d'une manipulation exogène visant à le(s) faire exprimer lesdites chaînes pro-alpha ou leurs dérivés avec des domaines qui présentent la même activité que les domaines propeptidiques C-terminaux mais ne s'associent pas avec le domaine propeptidique C-terminal des chaînes pro-alpha ou de leurs dérivés, lesquelles s'associent pour former ledit autre procollagène ou son dérivé.

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