C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/70 (2006.01) A61K 35/74 (2006.01) A61K 48/00 (2006.01)
Patent
CA 2701542
The invention relates to a vectorial system capable of delivering a nucleic acid of interest into eukaryotic target cells, said vectorial system including a recombinant non-pathogenic and non-replicative Escherichia coli bacterium (E. coli) having integrated into its chromosome, in a targeted manner and without any antibiotic marker, one or more genes imparting to said E. coli bacteria the capacity to penetrate into the cytoplasm of said eukaryotic target cells and to lyse the penetration vacuole. The present invention also relates to the use of such E. coli bacteria for the production of therapeutic compositions and their delivery without any purification step for preventing or treating diseases by vaccination or gene therapy.
La présente invention concerne un système vectoriel capable de délivrer dans des cellules cibles eucaryotes un acide nucléique d'intérêt, ce système vectoriel comprenant une bactérie Escherichia coli (E. coli) recombinante non pathogène et non réplicative ayant intégré de façon ciblée et sans marqueur antibiotique, dans son chromosome un ou plusieurs gènes conférant cette bactérie E. coli la capacité de pénétrer dans le cytoplasme de ces cellules cibles eucaryotes et de lyser la vacuole de pénétration. La présente invention comprend également l'utilisation de telles bactéries E. coli pour la production de compositions thérapeutiques et leur délivrance sans étape de purification destinées à la prévention ou au traitement de maladie par vaccination ou par thérapie génique.
Courvalin Patrice Marie Denis Paul
Goussard Sylvie Dominique
Grillot-Courvalin Catherine Liliane Andree
Centre National de La Recherche Scientifique (cnrs)
Institut Pasteur
Robic
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