C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/06 (2006.01) A01K 67/00 (2006.01)
Patent
CA 2381399
Disclosed herein are cultures of undifferentiated avian primordial germ cells/blastodermal cells. The cultures are capable of maintaining their undifferentiated characteristic and also their capability of expressing the EMA-1 epitope for extended periods when cultured in the presence of an isolate from avian navel (e.g. turkey navel extract). Methods of culturing such cultures using avian navel extracts, and culture media containing avian navel extract are also disclosed. Recombinant birds derived from these cultures are also disclosed. These cultures can be frozen for long-term preservation of germ line genomes.
La présente invention concerne des cultures de cellules blastodermiques/cellules germinales primordiales aviennes indifférenciées. Ces cultures peuvent conserver leur caractère indifférencié et leur capacité d'expression de l'épitope EMA-1 pendant longtemps lorsqu'elles dont cultivées en présence d'un isolat issu d'un ombilic avien (par exemple un extrait d'ombilic de dinde). Cette invention concerne aussi des techniques permettant de cultiver ces cultures par l'utilisation d'extraits d'ombilic avien, et des milieux de culture contenant ces extraits d'ombilic avien. L'invention concerne encore des oiseaux de recombinaison dérivés de ces cultures. Ces cultures peuvent être congelées pour obtenir une longue conservation des génomes des lignées cellulaires.
Bacon Larry D.
Hunt Henry D.
Tsai Herng
Wentworth Alice L.
Wentworth Bernard C.
Borden Ladner Gervais Llp
United States Department Of Agriculture
Wisconsin Alumni Research Foundation
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