Multiplex amplification of short tandem repeat loci

Details Multiplex amplification of short tandem repeat loci Multiplex amplification of short tandem repeat loci

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2251793

The invention is directed to the simultaneous amplification of multiple distinct genetic loci using PCR or other amplification systems to determine in one multiplex reaction the allels of each of the loci contained in the multiplex reaction. The genetic loci analyzed comprise the following: HUMvWFA31, HUMLIPOL, HUMBFXIII, HUMF13A01, HUMFESFPS, HUMTH01, HUMTPOX, HUMCSFIPO, D22S683, D20S481, D19S253, D17S1299, D17S1298, D16S753, D16S539, D16S490, D14S562, D14S548, D14S118, D13S317, D10S1239, D9S930, D7S820, D5S818, D4S2368, D3S1539.

L'invention concerne l'amplification simultanée, par PCR ou d'autres systèmes d'amplification, de multiples loci génétiques distincts, afin de déterminer en une seule réaction multiplex les allèles de chaque locus contenu dans ladite réaction. Les loci génétiques analysés sont les suivants: HUMvWFA31, HUMLIPOL, HUMBFXIII, HUMF13A01, HUMFESFPS, HUMTH01, HUMTPOX, HUMCSF1PO, D22S683, D20S481, D19S253, D17S1299, D17S1298, D16S753, D16S539, D16S490, D14S562, D14S548, D14S118, D13S317, D10S1239, D9S930, D7S820, D5S818, D4S2368, D3S1539.

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