C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/52 (2006.01) A01H 5/10 (2006.01) C12N 9/00 (2006.01) C12N 9/88 (2006.01) C12N 15/82 (2006.01)
Patent
CA 2390306
A method for altering the protein/fatty acid of seeds by introducing antisense gene, it comprises the following steps: (1) the cloning of genes of the phosphoenopyruvate carboxylase or acetyl-CoA carboxylase, or their fragments; (2) the construction of the corresponding antisense gene; (3) introducing antisense gene into the cell of oilseeds crop, so that the activity of the phosphoenopyruvate carboxylase or the acetyl-CoA carboxylase which is the key enzyme for protein synthesis will be changed, the flow direction of the biosynthetic co-substrate PEP could be ajusted, so that the protein/fatty acid composition of seeds will be altered. The application can be used for altering the protein/fatty acid composition of oilseeds crop.
L'invention concerne un procédé de régulation de la teneur en protéines/graisses dans une graine de plante faisant appel à un gène en sens inverse consistant à 1) cloner un gène de phosphoénolpyruvate carboxylase (PEPCase) ou un gène d'acétyl-CoA carboxylase (ACCase) ou son fragment, 2) construire un gène en sens inverse, 3) acheminer le gène obtenu dans une cellule adipeuse et modifier l'activité de limitation de vitesse de la PepCase lors de la synthèse protéique ou de l'ACCase lors de la synthèse d'acide carboxylique, à réguler le sens du PEP biosynthétisé de manière à modifier l'orientation pour réguler la teneur en protéines et en graisses des graines. L'invention peut trouver une application dans les cultures oléagineuses.
Chen Jinqing
Hu Zhanghua
Huang Ruizhi
Lang Chunxiu
Liu Zhihong
Gowling Lafleur Henderson Llp
Zhejiang Academy Of Agricultural Sciences
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