Analytical method using multiple virus labelling

Details Analytical method using multiple virus labelling Analytical method using multiple virus labelling

G - Physics

01

N

G01N 33/58 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2333463

The present invention relates to a method for detecting a target material in a sample, which method comprises the steps of: a) exposing a sample, expected to contain that target material, to at least two viruses which are capable of binding directly or indirectly with that target material so as to form a virally bound target material and to endow the virally bound target material with a distinctive property; and b) cultivating the product from stage <u>a</u> in the presence of an indicator material to which the viruses carried by the virally bound target material attach so as to cause the indicator material to adopt the distinctive property of the virally bound target material; and c) monitoring the presence or otherwise of virally attached indicator material. The invention also provides a kit for use in the method of the invention.

L'invention concerne un procédé de détection d'un matériau cible dans un échantillon. Ce procédé comprend les étapes suivantes: a) on expose de l'échantillon susceptible de contenir le matériau cible à au moins deux virus capables de se lier directement ou indirectement à ce matériau cible de manière à former un matériau cible à liaison virale et de conférer une propriété distinctive à ce matériau cible à liaison virale; b) on cultive le produit obtenu à l'étape <u>a</u> en présence d'un matériau indicateur sur lequel les virus portés par le matériau cible à liaison virale se fixent de manière que le matériau indicateur adopte la propriété distinctive du matériau cible à liaison virale; et c) on recherche la présence ou non du matériau indicateur à fixation virale. L'invention concerne également un kit convenant pour ce procédé.

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