Rescue of mumps virus from cdna

Details Rescue of mumps virus from cdna Rescue of mumps virus from cdna

C - Chemistry, Metallurgy

12

N

C12N 15/09 (2006.01) A61K 39/165 (2006.01) C07K 14/12 (2006.01) C12N 5/10 (2006.01) C12N 7/04 (2006.01) C12N 15/86 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2380799

This invention relates to a method for recombinantly producing, via rescue of mumps virus, a nonsegmented, negative-sense, single-stranded RNA virus, and immunogenic compositions formed therefrom. Additional embodiments relate to methods of producing the mumps virus as an attenuated and/or infectious virus. The recombinant viruses are prepared from cDNA clones, and, accordingly, viruses having defined changes, including nucleotide/polynucleotide deletions, insertions, substitutions and re-arrangements, in the place of the genome are obtained.

L'invention concerne un procédé recombinant de fabrication, par la récupération du virus des oreillons, d'un virus à ARN à brin unique à sens négatif non segmenté et de compositions immunogènes à partir de ce virus. Dans des modes de réalisation supplémentaires l'invention concerne la fabrication du virus des oreillons sous une forme atténuée et/ou infectieuse. Les virus recombinants sont préparés à partir de clones d'ADNc, ce qui permet d'obtenir des virus comprenant des suppressions, des insertions, des substitutions et des permutations de nucléotides/polynucléotides à l'endroit du génome.

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