Infection of eukaryotic cells with viruses in vitro

Details Infection of eukaryotic cells with viruses in vitro Infection of eukaryotic cells with viruses in vitro

C - Chemistry, Metallurgy

12

N

C12N 5/10 (2006.01) A61K 39/29 (2006.01) C12N 7/00 (2006.01)

Patent

CA 2393454

The invention related to a process for infecting eukaryotic cells with one or more virus species, preferentially hepatitis B or hepatitis C virus, as well as cell cultures infected by the same. To achieve this goal, plasma or serum obtained from individuals infected by viruses, preferentially hepadnaviridae and flaviviridae, is used an inoculum to infect established eukaryotic cell lines or primary cells, preferentially hepatocytes, which in turn produce viral particles. This process, the resulting infected cells and cell culture supernatant can be used in many situations, notably the screening of drug candidates, the detection of antibodies, the generation of a diagnostic kit and the production of vaccines.

L'invention se rapporte à un procédé pour infecter les cellules eucaryotes avec une ou plusieurs espèces de virus, notamment avec des virus de l'hépatite B et C, ainsi qu'aux cultures de cellules infectées par ces virus. Pour mettre en oeuvre ce procédé, on utilise le plasma ou le sérum obtenus chez des individus infectés par ces virus, appartenant de préférence aux virus ADN de l'hépatite ou aux flavivirus, en tant qu'inoculum pour infecter les lignées de cellules eucaryotes établies ou les cellules primaires, de préférence des hépatocytes, qui produisent à leur tour des particules virales. Ce procédé, les cellules infectées qui en résultent et la culture de cellules surnageante peuvent avoir de nombreuses applications, notamment dans le criblage de médicaments candidats, la détection d'anticorps, la génération d'un kit de diagnostic et la production de vaccins.

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