Method and apparatus for applying fluids to a biological sample

Details Method and apparatus for applying fluids to a biological sample Method and apparatus for applying fluids to a biological sample

G - Physics

01

N

G01N 33/53 (2006.01) G01N 1/30 (2006.01) G01N 1/31 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2573787

The invention is directed to a method of contacting a biological sample with a solution, comprising the steps of moving a curved surface wetted with the solution in proximity to the biological sample whereby the distance separating the wetted curved surface and the biological sample is sufficient to form a moving liquid meniscus layer between the two. The invention is also directed to an apparatus for contacting a biological sample suspected of containing a biomarker with a solution, comprising a platform for supporting a microscope slide having a biological sample thereon; a translating cap having a curved lower surface positioned above the platform, the curved lower surface being in proximity to a biological sample when in operation; means for moving the translating cap back and forth over the biological sample; and means for applying and removing liquid to and from the cap.

L'invention concerne un procédé de mise en contact d'un échantillon biologique avec une solution, comprenant les étapes de déplacement d'une surface incurvée mouillée avec la solution à proximité de l'échantillon biologique moyennant quoi la distance séparant la surface incurvée mouillée et l'échantillon biologique est suffisante pour former une couche de ménisque liquide mobile entre les deux. L'invention concerne aussi un appareil pour mettre en contact un échantillon biologique suspecté de contenir un biomarqueur avec une solution, comprenant une plate-forme pour supporter une lame microscopique ayant un échantillon biologique sur celle-ci; un couvercle coulissant ayant une surface inférieure incurvée positionnée au-dessus de la plateforme, la surface inférieure incurvée étant à proximité d'un échantillon biologique lors du fonctionnement; des moyens pour déplacer le couvercle coulissant en arrière et en avant au-dessus de l'échantillon biologique; et des moyens pour appliquer et enlever un liquide au/du couvercle.

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