C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 21/00 (2006.01) C12P 21/02 (2006.01)
Patent
CA 2159899
The present invention provides a novel high efficiency method for the cell free engineering and synthesis of protein. A novel method of the present invention comprises the steps of: preparing a cell free extract; separating out a ribosome fraction from said extract; incubating said ribosome fraction in the presence of a transcription/translation medium; and measuring the amount of protein synthesized. The method of the present invention may be used as a coupled transcription/translation system, a translation only system or a cell-free continuous flow system. Also provided are methods for synthesis of proteins and their correct folding using chaperone proteins.
Hardesty Boyd
Kramer Gisela
Kudlicki Wieslaw
Borden Ladner Gervais Llp
Research Development Foundation
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