Methods for absolute cell counting

Details Methods for absolute cell counting Methods for absolute cell counting

C - Chemistry, Metallurgy

12

M

C12M 1/34 (2006.01) C12M 1/26 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2374159

A method for determining the absolute cell count of an identifiable cell sub-population contained in a sample, the method which comprises the steps of introducing into an aliquot of the biological sample a pre-determined amount of a fluid reagent which comprises a known concentration of fixed and labeled cells and using flow cytometry to determine a count of the cells of the cell sub-population, and to determine a count of the labeled cells. The cell sub-population count and the labeled cell count is used to determine an absolute cell count of cell sub-population.

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