Magnetic particle capture of whole intact organisms from...

G - Physics – 01 – N

Patent

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G01N 33/543 (2006.01)

Patent

CA 2575451

A method includes providing a sample (10) containing at least one whole, intact particle or organism (20) in a container (15); creating a mixture (25) comprising the sample (10), at least one magnetically-responsive particle (30), and a remainder; and providing the mixture (25) with a pH of less than about 7Ø By providing the mixture (25) with a pH of less than about 7.0, alteration of the surface charge properties of at least the one magnetic particle (30) occurs, thereby causing the at least one whole, intact particle or organism (20) to become non-specifically bound to the at least one magnetically-responsive particle (30) to form a complex.

L'invention concerne un procédé comprenant la création d'un échantillon (10) contenant au moins une particule ou un organisme (20) entiers, intacts dans un récipient (15) ; la création d'un mélange (25) comprenant l'échantillon (10) et au moins une particule (30) sensible de manière magnétique, et un résidu ; la préparation d'un mélange (25) présentant un pH inférieur à 7Ø Lors de la préparation du mélange (25) comprenant un pH inférieur à 7.0, la modification des propriétés de charge de surface d'au moins une particule magnétique (30) apparaît, ce qui entraîne la liaison non spécifique d'au moins un organisme ou d'une particule (20) intacts et entiers à la particule (30) sensible de manière magnétique, ceci formant un complexe.

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