C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/11 (2006.01) C12Q 1/68 (2006.01) G01N 35/00 (2006.01)
Patent
CA 2416952
A set of oligonucleotide probes and method are disclosed for detecting a plurality of different target polynucleotides. The set includes a collection of different promiscuous probes each of which is capable of hybridising to a target sequence shared between at least two of the target polynucleotides. At least one target polynucleotide comprises at least one target sequence that is shared with one or more other target polynucleotides. A predefined combination of promiscuous probes is capable of hybridising to target sequences of said at least one target polynucleotide, wherein said predefined combination of probes provides specificity of detection of that target polynucleotide. Also disclosed are processes of identifying a set of target sequences for designing the set of oligonucleotide probes of the invention.
L'invention concerne un ensemble de sondes oligonucléotidiques ainsi qu'un procédé de détection de plusieurs polynucléotides cibles, différents. Cet ensemble comprend une collection de différentes sondes ubiquistes, chacune capable de s'hybrider à une séquence cible partagée par au moins deux polynucléotides cibles. Au moins l'un des polynucléotides cibles comprend au moins une séquence cible partagée avec au moins un autre polynucléotide cible. Une combinaison de sondes ubiquistes est capable de s'hybrider à des séquences cibles dudit polynucléotide cible, au moins, cette combinaison déterminée de sondes apportant la spécificité de détection du polynucléotide cible. L'invention concerne encore des procédés d'identification d'un ensemble de séquences cibles, servant à désigner l'ensemble de sondes oligonucléotidiques de l'invention.
Brown Roger William
Gibbs Adrian John
Gibbs Mark John
Fetherstonhaugh & Co.
The Australian National University
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