C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/85 (2006.01) A61K 47/48 (2006.01) C07K 14/47 (2006.01) C07K 19/00 (2006.01) C12N 5/10 (2006.01) C12N 15/11 (2006.01) C12N 15/62 (2006.01) C12Q 1/68 (2006.01) G01N 33/554 (2006.01) G01N 33/566 (2006.01) G01N 33/74 (2006.01)
Patent
CA 2405246
A method for selecting novel proteins such as growth hormone and antibody fragment variants having altered Minding properties for their respective receptor molecules is provided. The method comprises fusing a gene encoding a protein of interest to the carboxy terminal domain of the gene III coat protein of the filamentous phage M13. The gene fusion is mutated b form a library of structurally related fusion proteins that are expressed in low quantity on the surface of a phagemid particle. Biological selection and screening are employed to identify novel ligands useful as drug candidates. Disclosed are preferred phagemid expression vectors and selected human growth hormone variants. A method for selecting novel proteins such as growth hormone and antibody fragment variants having altered bind- ing properties for their respective receptor molecules is pro- vided. The method comprises fusing a gene encoding a protein of interest to the carboxy terminal domain of the gene III coat protein of the filamentous phage M13. The gene fusion is mu- tated to form a library of ratty rotated fusion proteins that are expressed in low quantity on the surface of a phagem- id particle. Biological selection and screening are employed to identify novel ligands useful as drug candidates, Disclosed are preferred phagemid expression vectors and selected human growth hormone variants.
Bass Steven
Garrard Lisa J.
Greene Ronald
Henner Dennis J.
Lowman Henry B.
Dennison Associates
Genentech Inc.
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