Promoter and plasmid system for genetic engineering

Details Promoter and plasmid system for genetic engineering Promoter and plasmid system for genetic engineering

C - Chemistry, Metallurgy

12

N

C12N 15/70 (2006.01) C12N 9/90 (2006.01) C12N 15/61 (2006.01) C12P 7/18 (2006.01)

Patent

CA 2481414

This invention provides a series of low-copy number plasmids comprising restriction endonuclease recognition sites useful for cloning at least three different genes or operons, each flanked by a terminator sequence, the plasmids containing variants of glucose isomerase promoters for varying levels of protein expression. The materials and methods are useful for genetic engineering in microorganisms, especially where multiple genetic insertions are sought.

La présente invention concerne une série de plasmides à faible nombre de copies comprenant des sites de reconnaissance de l'endonucléase de restriction utiles pour cloner au moins trois gènes ou opérons différents, chaque site étant flanqué d'un terminateur. Lesdits plasmides contiennent des variantes de promoteurs de glucose isomérase pour faire varier les taux d'expression protéique. Les matériaux et méthodes selon la présente invention sont utiles pour l'ingénierie génétique dans des micro-organismes, en particulier lorsque l'on cherche à opérer des insertions génétiques multiples.

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