Retroviral vector for the transfer and expression of genes...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/86 (2006.01) C12N 5/10 (2006.01) C12N 15/867 (2006.01)

Patent

CA 2143620

The invention relates to a recombinant vector for the cloning and/or expression and/or transfer of an exogenous nucleotide sequence, said vector being characterized in that it comprises any sequence contained in the fragment C-laI-PvuII comprising the nucleotides 7702 and 1527 of the sequence given in figure (1) and comprising the chain LTR comprised between the nucleotides 7842 and 144, the site PBS starting at the nucleotide 145, the encapsidation sequence comprised in the chain of 250 nucleotides following the end of the LTR sequence, said sequence being capable of controlling the cloning and/or expression and/or tranfer of the exogenous sequence, regardless of its transcriptional orientation with respect to the transcriptional orientation of the virus. The invention also relates to the utilisation of said vector for the transfer and/or cloning and/or expression of genes particularly for a genic therapy.

L'invention concerne un vecteur recombinant pour le clonage et/ou l'expression et/ou transfert d'une séquence de nucléotides exogène caractérisé en ce qu'il comprend toute séquence contenue dans le fragment claI-PvuII comprenant les nucléotides 7702 et 1527 de la séquence donnée à la figure 1 et comprenant l'enchaînement LTR compris entre les nucléotides 7842 et 144, le site PBS débutant au nucléotide 145, la séquence d'encapsidation comprise dans l'enchaînement de 250 nucléotides suivant la fin de la séquence LTR, ladite séquence étant capable de contrôler le clonage et/ou l'expression et/ou le transfert de la séquence exogène, quelle que soit son orientation transcriptionelle par rapport à l'orientation transcriptionelle du virus. Elle est relative à l'utilisation de ce vecteur pour le transfert et/ou le clonage et/ou l'expression de gènes notamment dans le cadre de la théraphie génique.

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