Separation of proteins based on isoelectric point using...

Details Separation of proteins based on isoelectric point using... Separation of proteins based on isoelectric point using...

B - Operations, Transporting

01

D

B01D 15/08 (2006.01)

Patent

CA 2616976

Proteins can be separated from mixtures, based on their pi values, through the use of a series of chromatographic materials, each comprising a solid buffer and an ion exchange resin. Each solid buffer generates a stable pH, such that passing proteins possess a net charge and can be separated by means of an appropriate ion exchanger. In this fashion, proteins from complex biological fluids can be separated for identification and study.

Il est possible de séparer des protéines de mélanges en fonction de leur PI à l'aide d'une série de matériaux chromatographiques comportant chacun un tampon solide et une résine échangeuse d'ions. Chaque tampon solide génère un pH stable, si bien que les protéines traversantes possèdent une charge nette et peuvent être séparées au moyen d'un échangeur d'ions approprié. On peut ainsi, séparer des protéines de fluides biologiques complexes pour les identifier ou les étudier.

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