Methods for carbon-centered radical mediated heavy hydrogen...

C - Chemistry – Metallurgy – 07 – K

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C07K 1/13 (2006.01)

Patent

CA 2376003

The solvent accessible reduced carbon atoms in a molecule of interest are labeled with heavy hydrogen. The carbon atoms are labeled under anaerobic, aqueous conditions by exposing the molecule of interest to a hydrogen atom abstractor in the presence of a heavy hydrogen source and a heavy hydrogen donor. If the hydrogen atom abstractor is hydroxyl radical, an electron scavenger source is provided. Optionally, an internal reference is provided to facilitate experimental reproducibility.

Des atomes de carbone réduits accessibles aux solvants, dans une molécule à analyser, sont marqués par de l'hydrogène lourd. Les atomes de carbone sont marqués dans des conditions anaérobies et aqueuses, par exposition de la molécule à analyser à un abstracteur d'atomes d'hydrogène, en présence d'une source d'hydrogène lourd et d'un donneur d'hydrogène lourd. Si l'abstracteur d'hydrogène est un radical hydroxyle, une source d'accepteurs d'électrons est fournie. Eventuellement, une référence interne est fournie pour faciliter la reproductibilité de l'expérience.

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