Live attenuated salmonella vaccine

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 1/20 (2006.01) A61K 39/108 (2006.01) A61K 39/112 (2006.01) A61P 31/04 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2643740

The present invention is related to double and triple attenuated mutant strains of a bacterium infecting veterinary species such as Salmonella enterica and/or (a pathogenic) Escherichia coli. The mutants of the invention contain at least one first genetic modification and at least one second genetic modification, said first modification in one or more motility genes, and said second modification in one or more genes involved in the survival or the proliferation of the pathogen in the host. The present invention further relates to live attenuated vaccines based on such mutants for preventing amongst others Salmonellosis and/or an infection by an E. coli pathogen in a veterinary species.

La présente invention concerne des souches mutantes atténuées doubles et triples d'une bactérie infectant des espèces animales, telle que Salmonella enterica et/ou Escherichia coli (un agent pathogène). Les mutants selon cette invention comportent au moins une première modification génétique et au moins une seconde modification génétique, la première modification génétique dans un ou plusieurs gènes de la motilité et la seconde modification génétique dans un ou plusieurs gènes impliqués dans la survie et la prolifération de l'agent pathogène chez l'hôte. Cette invention concerne également des vaccins vivants atténués à base de tels mutants, conçus pour prévenir, entre autres, une salmonellose et/ou une infection par un agent pathogène E. coli chez une espèce animale.

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