Apparatus and methods for controlling cellular development

Details Apparatus and methods for controlling cellular development Apparatus and methods for controlling cellular development

C - Chemistry, Metallurgy

12

N

C12N 13/00 (2006.01) C12N 5/071 (2010.01) A61K 38/17 (2006.01) A61K 41/00 (2006.01) A61N 5/06 (2006.01)

Patent

CA 2728402

According to one aspect and example, a method for facilitating cellular interactions in biological tissue provides controllable activation of a selected type of stem cell among a plurality of cell types present in the tissue. The method includes various steps including the introduction of a microbial opsin into a region of the tissue that includes a selected type of stem cell, by expressing the microbial opsin in the stem cell. A light source is then introduced near the stem cell, and the light source is used to controllably activate thejight source to direct pulses of illumination from the light source to the selected type of stem cell, for selectively controlling the growth and development of the stem cell in a manner that is independent of the growth and development of the other types of cells.

Selon un aspect et un exemple de l'invention, un procédé pour faciliter les interactions cellulaires dans des tissus biologiques permet d'obtenir une activation pouvant être commandée d'un type sélectionné de cellule souche parmi une pluralité de types de cellules présentes dans les tissus. Le procédé comprend plusieurs étapes parmi lesquelles on peut citer l'introduction d'une opsine microbienne dans une région des tissus qui comprend un type sélectionné de cellule souche, par expression de l'opsine microbienne dans la cellule souche. Une source lumineuse est ensuite introduite à proximité de la cellule souche, et la source lumineuse est activée de manière commandée pour diriger des impulsions d'éclairage de ladite source lumineuse vers le type sélectionné de cellule souche, afin de commander de manière sélective la croissance et le développement de la cellule souche indépendamment de la croissance et du développement des autres types des cellules.

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