Lentivirus pseudotyped with influenza hemagglutinin and...

A - Human Necessities – 61 – K

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A61K 39/145 (2006.01) C12N 15/867 (2006.01)

Patent

CA 2673994

Highly effective pseudotyping of lentiviral vector with influenza HA, NA and M2 packaging gene constructs at the proper ratios. Lentivirus vector pseudotyped with influenza HA, especially pseudotyped with H5 and neuraminidase. Methods of inducing immune responses to influenza antigens or for transducing genes into cells to which influenza antigens bind using such lentivirus vectors. Methods for screening drugs which inhibit influenza infection using lentivirus pseudotyped with HA.

La présente invention concerne un pseudotypage extrêmement efficace du vecteur lentiviral avec les virus de la grippe HA, NA et M2 encapsidant des gènes chimères dans les proportions adéquates. L'invention concerne plus particulièrement un vecteur de lentivirus pseudotypé avec la grippe HA, en particulier pseudotypé avec H5 et une neuraminidase. L'invention concerne en outre des procédés d'induction de réponses immunes à des antigènes de grippe, ou de transduction de gènes dans des cellules auxquelles des antigènes de la grippe se lient en utilisant de tels vecteurs de lentivirus. L'invention concerne également des procédés de sélection de médicaments qui inhibent une telle infection de grippe en utilisant un lentivirus pseudotypé avec HA.

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