Improved viral purification methods

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 7/02 (2006.01) A61K 39/15 (2006.01) C07K 14/14 (2006.01)

Patent

CA 2482512

The present invention is directed to an improved method of purifying virus, particularly reovirus. Infectious virus can be extracted from a cell culture with a detergent to produce high titers of virus, and the virus can then be purified by simple steps such as filtration and column chromatography. Viruses and compositions comprising the viruses prepared according to the present invention are also provided.

L'invention porte sur un procédé amélioré de purification de virus et en particulier de rétrovirus, selon lequel les virus infectieux sont extraits d'une culture de cellules au moyen d'un détergent de manière à obtenir des titres élevés de virus, dont les virus peuvent être purifiés par des méthodes simples telles que le filtrage ou la chromatographie sur colonne. L'invention porte également sur virus ainsi obtenus, et sur des compositions les comprenant.

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