Polynucleotides for the detection of escherichia coli...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/31 (2006.01) C07H 21/00 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2566949

Polynucleotide primers and probes for the specific detection of E. coli 0157:H7 and/or E. coli 0157:NM which produces verotoxin in samples are provided. The primers and probes can be used in real time diagnostic assays for rapid detection of E. coli 0157:H7 and/or E. coli 0157:NM which produces verotoxin. The primers and probes can be used in real-time diagnostic assays for rapid detection of E. coli 0157:H7 and/or E. coli 0157:NM which produces verotoxin in a variety of situations and are capable of distinguishing E. coli 0157:H7 and /or E. coli 0157:NM which produces verotoxin from other E. coli strains. Kits comprising the primers and probes are also provided.

L'invention concerne des amorces et sondes polynucléotidiques servant à détecter de manière spécifique E. coli <I>O157:H7</I> et/ou E. coli O157:NM qui produisent de la vérotoxine dans des échantillons. Les amorces et sondes peuvent être utilisées dans des méthodes diagnostiques en temps réel pour détecter rapidement E. coli <I>O157:H7</I> et/ou E. coli O157:NM qui produisent de la vérotoxine. Les amorces et sondes peuvent être utilisées dans des méthodes diagnostiques en temps réel pour détecter rapidement E. coli <I>O157:H7</I> et/ou E. coli O157:NM qui produisent de la vérotoxine dans une variété de situations et peuvent faire la distinction entre E. coli O157:H7 et/ou E. coli O157:NM qui produisent de la vérotoxine, et d'autres souches d'E. coli. La présente invention concerne également des trousses renfermant lesdites amorces et sondes.

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