Insertion of dna by modified transposons

Details Insertion of dna by modified transposons Insertion of dna by modified transposons

C - Chemistry, Metallurgy

12

N

C12N 15/66 (2006.01) A61K 39/02 (2006.01) C07K 14/245 (2006.01) C07K 14/445 (2006.01) C12N 15/63 (2006.01) C12N 15/70 (2006.01) C12N 15/74 (2006.01) C12N 15/90 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2052324

31638 INSERTION OF DNA BY MODIFIED TRANSPOSONS Abstract of the Disclosure DNA constructs for the introduction of a DNA sequence into the constituent DNA of a prokaryote, and methods of use. The DNA construct includes an express- ible gene encoding a transposage protein, linked in cis to a transposable cassette. The transposable cassete includes a pair of transposase recognition sequences flanking the DNA sequence. The gene encoding the transposase protein is not flanked by the transposase recognition sequences.

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