Synthetic gene coding for human parathyroid hormone

Details Synthetic gene coding for human parathyroid hormone Synthetic gene coding for human parathyroid hormone

C - Chemistry, Metallurgy

12

N

C12N 15/16 (2006.01) C07K 14/635 (2006.01) C12N 15/70 (2006.01)

Patent

CA 2052396

Disclosed are (1) a DNA containing a synthetic gene for expression of human parathyroid hormone represented by the following DNA sequence: TCTGTG TCCGAGATTC AGTTAATGCA TAACCTTGGC AAACATTTGA ACTCCATGGA GCGTGTAGAA TGGCTGCGTA AGAAGTTGCA GGATGTGCAC AATTTTGTTG CCTTAGGTGC CCCATTGGCT CCTCGTGATG CTGGTTCCCA AAGACCACGT AAAAAGGAAG ACAATGTCTT AGTTGAGAGC CATGAAAAAT CCCTAGGCGA GGCAGACAAG GCCGATGTGA ATGTATTAAC TAAAGCTAAA TCCCAG (2) a method for producing the DNA described in (1), which comprises enzymatically ligating a plurality of oligodeoxynucleotides to one another to form the DNA and inserting the DNA into a vector if necessary, (3) a transformant transformed by the DNA described in (1), and (4) a method for producing human parathyroid hormone which comprises cultivating the transformant described in (3), accumulating human parathyroid hormone in a culture medium, and collecting the same, whereby hPTH can be allowed to express in large amounts in a system using E. coli as a host.

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