Novel method for cloning variable domain sequences of...

C - Chemistry – Metallurgy – 12 – Q

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C12Q 1/68 (2006.01) A61K 38/00 (2006.01) C07H 21/02 (2006.01) C07K 16/00 (2006.01) C12N 1/20 (2006.01) C12N 15/00 (2006.01) G01N 33/536 (2006.01)

Patent

CA 2450217

The present invention relates to a non-PCR (polymerase chain reaction) process, particularly a transcription-based amplification method, for amplifying and cloning sequences containing a variable domain sequence such as an immunoglobulin variable domain sequence from the immunological gene repertoire. The present invention comtemplates the expression of antibody library in either in an in vivo expression vector or in an in vitro transcription/translation system. Isolation of a gene coding for a receptor having the ability to bind a preselected ligand and receptors produced by the gene isolated by the method is also contemplated.

La présente invention concerne un procédé non PCR (amplification par polymérase), en particulier un procédé d'amplification à base de transcription, permettant d'amplifier et de cloner des séquences contenant une séquence à domaine variable et notamment une séquence à domaine variable de l'immunoglobuline à partir du répertoire de gènes immunologiques. La présente invention concerne ainsi l'expression d'une échantillothèque d'anticorps, soit dans un vecteur d'expression in vivo, soit dans un système de transcription-traduction in vitro. L'invention concerne enfin l'isolement d'un gène codant pour un récepteur capable de se lier à un ligand défini et des récepteurs produits par le gène isolé selon le procédé.

LandOfFree

Say what you really think

Search LandOfFree.com for Canadian inventors and patents. Rate them and share your experience with other people.

Rating

Novel method for cloning variable domain sequences of... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Novel method for cloning variable domain sequences of..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Novel method for cloning variable domain sequences of... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFCA-PAI-O-1503509

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.