Methods for ranking cellular images

Details Methods for ranking cellular images Methods for ranking cellular images

G - Physics

01

N

G01N 15/10 (2006.01) G01N 15/14 (2006.01) G06T 7/00 (2006.01)

Patent

CA 2662859

The methods described in this invention are used to analyze images of circulating tumor cells (CTC). Images are acquired from a number of platforms, including multiparameter flow cytometry, the CellSporter fluorescent microscopy imaging system and CellTracks Analyzer. These images are then ranked based on various properties and are presented to the user in order of most likely to least likely positive CTC events. The ranking method is useful to diagnose, monitor, and screen disease based on circulating rare cells, such as malignancy as determined by CTC.

L'invention concerne des procédés qui sont utilisés pour analyser des images de cellules tumorales circulantes (CTC). Les images sont acquises à partir d'un certain nombre de plateformes, comprenant une cytométrie de flux à multiples paramètres, le système d'imagerie par microscope fluorescent CellSporter et l'Analyseur CellTracks. Ces images sont ensuite classées en fonction de diverses propriétés, et sont présentées à l'utilisateur dans un ordre d'événements CTC allant de la probabilité d'être positif la plus haute à la plus basse. Le procédé de classement est utile pour diagnostiquer, surveiller et cribler une maladie en fonction de cellules rares circulantes, telle qu'une tumeur telle que déterminée par CTC.

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