Process for producing recombined protein

Details Process for producing recombined protein Process for producing recombined protein

C - Chemistry, Metallurgy

12

P

C12P 21/02 (2006.01) C12N 15/09 (2006.01) C12N 15/70 (2006.01)

Patent

CA 2417572

It is intended to provide a process for efficiently producing a recombined protein free from the N-terminal methionine. In culturing a host transformed by an expression vector for expressing a gene-modified protein, the dissolved oxygen concentration in the liquid culture medium is controlled to about 1 ppm or lower. Thus, the ratio of the expression of a recombined protein free from the N-terminal methionine is elevated compared with the case wherein no such control is performed.

L'invention concerne un procédé de production efficiente d'une protéine recombinée exempte de méthionine N-terminale. La culture d'un hôte transformé par un vecteur d'expression pour exprimer une protéine modifiée par un gène permet de réguler à environ 1 mg/l au maximum la concentration d'oxygène dans le milieu de culture liquide. Ainsi, le ratio de l'expression d'une protéine recombinée exempte de méthionine N-terminale est augmenté par comparaison avec le cas où une telle régulation n'est pas effectuée.

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