A method for displaying proteins

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/62 (2006.01) C07K 14/245 (2006.01) C07K 16/00 (2006.01) C12N 1/21 (2006.01) C12N 15/10 (2006.01) C12N 15/70 (2006.01) C12Q 1/02 (2006.01) C12Q 1/70 (2006.01) G01N 33/554 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2169358

The present invention relates to a fusion pro- tein, comprising a pilin protein or a portion thereof and a heterologous polypeptide (target protein). In a preferred embodiment it relates to a method for displaying the target protein on the outer surface of a bacterial host cell capable of forming pilus. In certain embodiments, it is desirable that the pilus is a receptor for bacteriophage attachement and infec- tion. The F pilus is preferred.

L'invention concerne une protéine de fusion comprenant une protéine piline ou une partie de celle-ci ainsi qu'un polypeptide hétérologue (protéine cible). Dans un mode de réalisation préféré, l'invention a trait à un procédé de présentation de la protéine cible sur la surface extérieure d'une cellule hôte bactérienne capable de former un pilus. Dans certains modes de réalisation, il est souhaitable que le pilus soit un récepteur de fixation de bactériophage et d'infection. Le pilus F est préféré.

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