Plant acetyl-coa carboxylase polypetide and gene

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/52 (2006.01) C12N 5/10 (2006.01) C12N 9/00 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2048040

ABSTRACT A purified, biotin-containing polypeptide with a molecular weight of about 50 kilodaltons is disclosed herein. The polypeptide is a subunit of plant acetyl-CoA carboxylase, which catalyzes the carboxylation of plant acetyl-CoA to form plant malonyl-CoA. Modified polypeptides are also disclosed herein as well as methods for obtaining the polypeptides. Also disclosed herein are DNA sequences coding for the polypeptides and methods of obtaining a preferred cDNA sequence by screening a cDNA expression library with antibodies to the 50kD polypeptide.

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