Materials and methods for amplifying and enhanced...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/10 (2006.01) C12N 5/10 (2006.01) C12N 15/11 (2006.01) C12N 15/29 (2006.01) C12N 15/67 (2006.01) C12N 15/82 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2330730

Products and methods for amplifying target nucleic acids using cells derived from plants are disclosed. Also disclosed are methods for improving transcription of target nucleic acids beyond expectations based solely on target copy number, and the recovery of such expression products. The products include nucleic acids containing a plant-active Amplification Promoting Sequence (APS) and the methods exploit these products in amplifying target nucleic acids. The methods of the invention minimize operator intervention and exploit solar energy and the minimal nutrient needs of photoautotrophic organisms to provide inexpensive and indefinitely sustainable methods for producing a variety of amplified target nucleic acids and encoded products such as polypeptides.

L'invention concerne des produits et des méthodes pour l'amplification d'acides nucléiques cibles au moyen de cellules dérivées de plantes. Elle porte encore sur des méthodes d'amélioration de la transcription d'acides nucléiques cibles, au-delà des résultats escomptés, uniquement quant au nombre de copies cibles, et de récupération desdits produits d'expression. Lesdits produits comprennent des acides nucléiques contenant une séquence favorisant l'amplification active au niveau de la plante et lesdits procédés permettent d'exploiter ces produits dans l'amplification d'acides nucléiques cibles. Les procédés de l'invention permettent de minimiser l'intervention de l'opérateur et d'exploiter l'énergie solaire et les besoins nutritifs minimaux d'organismes photoautotrophes, pour la mise en oeuvre de procédés économiques et renouvelables à l'infini, de production d'une grande variété d'acides nucléiques amplifiés et de produits codés, tels que des polypeptides.

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