Selective amplification system using q beta replicase

Details Selective amplification system using q beta replicase Selective amplification system using q beta replicase

C - Chemistry, Metallurgy

07

H

195/1.12, 150/8.

C07H 21/04 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2040503

-42- SELECTIVE AMPLIFICATION SYSTEM USING Q BETA REPLICASE Abstract of the Disclosure Mutant forms of midivariant RNA are disclosed which are replicated efficiently by the enzyme Q.beta. replicase in the presence of an agent(s) which is useful for the real time, quantitative detection of the accumulating RNA products. This agent(s) also suppresses the replication of non-mutant wild type midivariant RNA. The mutant MDV RNAs, when modified by the addition of specific probe sequences, retain their replication properties in the presence of the detection agent. This method allows the replication to be monitored using a real time mode thereby providing a reliable method for monitoring the kinetics of the reaction, and determining the initial level of amplified RNA.

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