Assays and probes with enzyme labels

Details Assays and probes with enzyme labels Assays and probes with enzyme labels

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) G01N 33/535 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2269840

Probes comprise S1 and P1 nuclease (as an enzyme label) linked to a specific binding member such as a nucleotide sequence or an antibody. Such probes are useful for sandwich assays. As compared with known probes using alkaline phosphatase as a label, advantages include relative insensitivity to phosphate and elevated temperature and reduced risk of nonspecific binding.

Des sondes comprenant les nucléases S1 et P1 (comme enzymes marqueurs), liées à un élément de liaison spécifique tel qu'une séquence nucléotidique ou un anticorps, sont utiles pour les méthodes de dosage en sandwich. Par comparaison avec les sondes connues utilisant comme marqueurs les phosphatases alcalines, ces sondes ont pour avantages une insensibilité relative aux phosphates et à l'élévation de température, ainsi qu'un risque moindre de liaison non spécifique.

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