Methods of detecting an analyte in a sample

Details Methods of detecting an analyte in a sample Methods of detecting an analyte in a sample

C - Chemistry, Metallurgy

12

Q

C12Q 1/48 (2006.01) C07K 16/46 (2006.01) C12N 9/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2595673

The invention relates to methods for detecting an analyte in a sample. The methods rely on the activity of polymerases upon polynucleotide substrates which are linked to a molecule, for example an antibody, which binds the analyte. Activity of the polymerases can be detected by the incorporation of suitably labelled nucleotides, and/or the incorporation of hapten conjugated nucleotides capable of binding a suitably labelled ligand of the hapten.

La présente invention concerne des procédés pour la détection d~une substance à analyser dans un échantillon. Les procédés reposent sur l~activité de polymérases sur des substrats polynucléotidiques liés à une molécule, par exemple un anticorps, qui se lie à la substance à analyser. L~activité des polymérases peut être détectée par l'incorporation de nucléotides convenablement marqués, et/ou l~incorporation de nucléotides conjugués à un haptène capables de lier un ligand convenablement marqué de l'haptène.

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