Methods of islet separation during isolation

Details Methods of islet separation during isolation Methods of islet separation during isolation

A - Human Necessities

61

K

A61K 35/39 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2637281

The present invention relates to methods for improving the viability and recovery of islets that are separated from a donor organ for subsequent transplantation. Tn a preferred embodiment, the islets are separated from a donor pancreas and trans- planted into the liver of a diabetic patient. A protective agent is mixed with a separating solution to form a protective separating solution which is utilized to separate the islets cells from other cells after the donor pancreas is digested with an enzyme. The protective agent increases the viscosity of the solution during the separation process, creating a cushioning effect of islets, thereby enhancing their viability and health so that the islets' membranes may withstand a vigorous isolation procedure such as the Edmonton Protocol.

La présente invention concerne des méthodes pour améliorer la viabilité et la récupération d'îlots qui sont séparés d'un organe donneur pour transplantation ultérieure. Dans un mode de réalisation préféré, les îlots sont séparés d'un pancréas donneur et transplantés dans le foie d'un patient diabétique. Un agent protecteur est mélangé à une solution séparatrice pour former une solution séparatrice protectrice utilisée pour séparer les cellules des îlots des autres cellules après digestion du pancréas donneur par une enzyme. L'agent protecteur augmente la viscosité de la solution pendant le processus de séparation, créant un effet amortissant sur les îlots, améliorant ainsi la viabilité et la santé desdits îlots de sorte que les membranes des îlots puissent supporter un vigoureux processus d'isolement tel que le Protocole Edmonton.

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